Genetics · Module 7 · DNA replication and recombination

DNA Replication Lab

Race a bacterial genome against a eukaryotic one, resolve Holliday junctions into crossovers or non-crossovers, practise six kinds of replication and recombination problems with animated worked solutions, sort the replisome proteins and order the steps, and use it all on real cases. Nothing here is graded.

Part 1: Replication Race

Unreplicated DNAReplicated (bubbles)ForkOrigin that firedOrigin copied passively (dormant)
Race readouts for both genomes
MeasureLane ALane B

Simplifications: every fork moves at one steady speed and forks never stall; origins are evenly spaced. "All at once" gives the minimum time t = G / (2 n v). "Staggered" fires origins at random times over a window and lets late origins be copied passively, like dormant origins. When there are more than 24 origins, the lane shows a window of 24 neighbouring replicons; every replicon behaves the same way. Okazaki fragments are estimated as genome size divided by fragment length; primers add 2 per fired origin for the leading strands. Real S phases are longer because origins fire in a timed program.