Microbial Metabolism Lab

Part 1: Cell Energy Simulator

Glucose Pyruvate ATP NADH (full carrier) Electron Active route

1. Choose the organism

2. Oxygen

Add nitrate (NO₃⁻) to the broth

Cell energy readouts
Measure	Value

Challenges

A teaching model. ATP counts use the classic textbook values (38 aerobic, 2 fermentation); the anaerobic respiration value (20) is a round middle figure, since real yields vary.

Part 2: Enzyme Simulator

Enzyme Substrate Competitive inhibitor Noncompetitive inhibitor Product Reaction rate

1. Choose the enzyme

2. Conditions

Temperature 37 °C

pH 7.0

Substrate amount 40

Competitive inhibitor 0

Noncompetitive inhibitor 0

Enzyme simulator readouts
Measure	Value

Challenges

A teaching model with rounded numbers. Real enzymes differ in their optimum, their sensitivity and how fast they denature.

Part 3: Unknowns Bench

Unknown 1 of 7Questions solved 0First try 0

Red: no acid Yellow: acid Pink: alkaline Blue: citrate + Hot pink: urease + +/− positive or negative

Run tests (each takes a simulated day of incubation)

Test results recorded so far
Test	Result	Test	Result

Questions

Wrong answers are fine here. Each one tells you why.